Thp-1 macrophage-like cells
WebTHP-1 cells (human monocytic cell line) were cultured in 6-well cell culture plates (1×10 6 cells per well) and maintained in complete RPMI 1640 medium at 37°C. For differentiation … WebMonociles and their macrophage and dendritic-cell progeny serve three main functions the the immune system. Those have phagocytosis, antigen showcase, and cytokine …
Thp-1 macrophage-like cells
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WebJul 29, 2008 · Although monocytic leukemia cell lines such as THP-1, KG-1, K562, monomac-6, and U-937 had originally been predicted to be good candidates for differentiation into DC-like cells, these leukemia cell lines have been demonstrated to be poor models for DC differentiation, most likely as they are already developed too far along … WebThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease-19 (COVID-19). The spike protein (S) of SARS-CoV-2 plays a crucial role in mediating viral infectivity; hence, in an extensive effort to
WebImmunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-α expression. Author links open … WebApr 24, 2024 · In parallel, THP-1 monocytes were differentiated into macrophage-like cells using PMA. When SMCs reached confluence ECs were seeded on the upper side of transwell inserts and allowed to adhere for 24 h. Inserts were then transferred into plates containing LPS stimulated adherent macrophage-like THP-1 cells.
WebMar 3, 2024 · Briefly, for migration assay, 200 μL of cell suspensions without FBS (for THP-1 cells, 1 × 10 5 cells pretreated with 100 ng/mL PMA (Sigma-Aldrich, P8139) for 24 hours … WebWe also found the same trends for CARINH and IL18BP levels upon the knockdown and overexpression of IRF1 in THP-1 cells (Fig. 5j, k), again supporting the existence of a …
WebApr 4, 2024 · Following differentiation of the THP-1 cells, MERTK upregulation was confirmed by western blot and flow cytometry. In a similar fashion, treatment with of murine bone marrow derived monocytic ...
WebThe invention discloses a construction method of a mononuclear macrophage model based on HIV-1 (human immunodeficiency virus type 1) mass infection duplication, which comprises the following steps: designing a pair of identification sequences for SAMHD1 gene CDS1, and constructing a TALEN plasmid pair; transfecting the TALEN plasmid pair … the national hotel block island riWebApr 13, 2024 · HIGHLIGHTS. who: Maria Rynikova and colleagues from the Department of Animal Physiology, Faculty of Science, Pavol Jozef Safarik University in have published … how to do a sharpshooterWebApr 9, 2024 · Type i cystatin derived from fasciola gigantica suppresses macrophage-mediated inflammatory responses. ... (dexa) was used as a standard anti-inflamstudy the … how to do a shiny only nuzlockeWebTHP-1 cells were treated with PMA, IFN-γ + LPS and IL-4 + IL-13, respectively, and affixed with 4% polyoxymethylene (Sigma-Aldrich) at 4°C for 15 min. ... Zhang F, Wang H, Wang X, … how to do a shift shutdownWebLoss of ME-1 in THP-1 cells was also associated with reduced efferocytosis. ME-1 is a target gene of NRF2 (nuclear factor erythroid 2–related factor 2), and the authors have … how to do a shirt in robloxWebSep 4, 2024 · In the co-culture system, THP-1 cells were induced to the M2-like state by SW480 cells. The level of miR-148a negatively correlated with the levels of M2-related cytokines. ... and the macrophage cells that migrated to the lower surface were fixed with formalin and stained with 0.1% crystal violet. how to do a shiplap accent wallWebThe target sequence for the COX-2 siRNA was bases 291?C313 of NM000963.1. (5??-aactgctcaacaccggaattttt ... Tumor-associated macrophage-induced invasion and … how to do a shed roof