2024 Pcra helicase

Pcra helicase

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Splet12. jun. 2015 · In this study we show that without the essential Bacillus subtilis accessory DNA helicase, PcrA, the replication machinery slows down at certain regions of the chromosome in a transcription-dependent manner. PcrA is essential to life but incomplete depletion of PcrA only partially inhibits cell survival. SpletIordanescu S. Characterization of the Staphylococcus aureus chromosomal gene pcrA, identified by mutations affecting plasmid pT181 replication. Mol Gen Genet. 1993 Oct;241 (1-2):185-92. Petit MA, Dervyn E, Rose M, Entian KD, McGovern S, Ehrlich SD, Bruand C. PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in ...

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Splet01. feb. 2009 · PcrA helicase then binds to the ssDNA and begins unwinding. RepD has been shown to increase PcrA helicase activity; in the absence of RepD PcrA is a poor … Splet12. jun. 2015 · PcrA’s ATPase and helicase activities are required for conflict mitigation In vitro studies of PcrA have demonstrated that its helicase and ATPase activities are … mansfield classifieds

Biochemical Characterization of the Staphylococcus …

Splet04. jun. 2012 · The ATPase cycle of PcrA helicase and its coupling to translocation on DNA. J. Mol. Biol., 392, 1020-1032 (2009) CrossRef PubMed CAS Google Scholar Smith, R.H.; Kotin, R.M.: The Rep52 gene product of adeno-associated virus is a DNA helicase with 3’-to-5’ polarity. J. Virol., 72, 4874-4881 (1998) PubMed ... Splet23. okt. 2024 · Polymerase Chain Reaction (PCR) is an essential method in molecular diagnostics and life sciences. PCR requires thermal cycling for heating the DNA for strand separation and cooling it for replication. The process uses a specialized hardware and exposes biomolecules to temperatures above 95 °C. Splet16. okt. 2013 · UvrD-like helicases play diverse roles in DNA replication, repair and recombination pathways. An emerging body of evidence suggests that their different cellular functions are directed by interactions with partner proteins that target unwinding activity to appropriate substrates. kots config

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SpletPurified PcrA helicase supported the in vitro replication of plasmid pT181. It had ATPase activity that was stimulated in the presence of single-stranded DNA. Unlike many … Splet15. jan. 2024 · Nucleic-acid-processing motor enzymes such as helicases, polymerases and translocases that move along DNA or RNA play vital roles in genome replication, expression and maintenance ( 1–6 ). The nucleic-acid (NA) sequence has been shown to regulate important properties of some of these enzymes. mansfield classlink loginSpletPcrA helicase, a prototype ATP-driven molecular motor Authors Markus Dittrich 1 , Klaus Schulten Affiliation 1 Theoretical and Computational Biophysics Group, Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA. PMID: 16962966 DOI: 10.1016/j.str.2006.06.017 Abstract mansfield civic centre room hire

"SpletTte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis.It is active on a wide range of DNA substrates and, along with its thermostability (active to 70°C), Tte UvrD Helicase has been demonstrated … " - Pcra helicase

Pcra helicase

Splet02. apr. 1999 · We have determined two different structures of PcrA DNA helicase complexed with the same single strand tailed DNA duplex, providing snapshots of … Splet31. jul. 2007 · DNA helicases are ATP driven molecular motors involved in all aspects of DNA metabolism, including transcription, replication and recombination. They catalyze …

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Splet17. apr. 2024 · Bacillus subtilis PcrA Helicase Removes Trafficking Barriers Authors María Moreno-Del Álamo 1 , Begoña Carrasco 1 , Rubén Torres 1 , Juan Carlos Alonso 1 … Splet16. okt. 2013 · The PcrA-RNAP interaction is direct and independent of nucleic acids or additional mediator proteins. A disordered but highly conserved C-terminal region of …

Splet01. mar. 1999 · Whereas L3 stimulates the PcrA helicase activity by promoting co-operative PcrA binding onto its DNA substrate, RepD stimulates the PcrA helicase activity by increasing the processivity of the enzyme and enables PcrA to displace DNA from a nicked substrate. The implication of these results is that PcrA is the helicase recruited into the ... Splet17. jul. 2001 · In this model, PcrA helicase is functional as a monomer that separates duplex DNA in a processive manner by coupling two distinct activities. First, PcrA translocates unidirectionally along single-stranded DNA (ssDNA) by virtue of a ssDNA tracking motor that is powered by ATP hydrolysis.

SpletPlasmid pET-11b_PcrA_M6_H93A from Dr. Taekjip Ha's lab contains the insert Engineered helicase PcrA_M6 and is published in Nat Commun. 2024 Oct 23;13(1):6312. doi: 10.1038/s41467-022-34076-0. This plasmid is available through Addgene. SpletBy means of in silico mutation studies, we identified the residue Q254 as being crucial for the coupling of ssDNA translocation to the actual catalytic event. Based on the present …

Splet13. dec. 2001 · Use of the fluorescent base analogue 2-aminopurine has provided a direct demonstration of the translocation of PcrA helicase toward the 5‘-end of single-stranded DNA. Single 2-aminopurine bases are introduced into otherwise standard oligonucleotides and produce a fluorescence signal when PcrA reaches their position. We demonstrate …

Splet08. avg. 2013 · The superfamily 1 helicase, RecD2, is a monomeric, bacterial enzyme with a role in DNA repair, but with 5′-3′ activity unlike most enzymes from this superfamily. Rate constants were determined for steps within the ATPase … kotschs whitehall paSplet01. avg. 1999 · DNA helicases are ubiquitous enzymes involved in almost all aspects of DNA metabolism. They hydrolyse ATP and use the associated free energy change to … kotsch\u0027s market whitehall paSpletAbstract Recently determined crystal structures of PcrA helicase complexed with a DNA substrate have revealed details of the helicase mechanism. PcrA and UvrD helicases have been shown to be functional as monomers, challenging previous suggestions that all helicases are required to be oligomeric. mansfield city tax rateSplet02. apr. 1999 · We have determined two different structures of PcrA DNA helicase complexed with the same single strand tailed DNA duplex, providing snapshots of different steps on the catalytic pathway. One of the structures is of a complex with a nonhydrolyzable analog of ATP and is thus a "substrate" complex. mansfield city texasSpletIn the case of the monomeric DNA helicase PcrA, these properties can be uncoupled by mutation, as certain PcrA mutants lack duplex unwinding but maintain DNA translocation (Soultanas et al. 2000). In addition, PcrA can translocate along a single strand of DNA ( Dillingham et al. 2000 , 2002 ), and the crystal structure of PcrA shows the binding ... kotsch\u0027s meat market - whitehallSpletPcrA helicase showed an origin-specific unwinding activity with supercoiled plasmid pT181 DNA that had been nicked at the origin by RepC. We also … kots constructionSplet开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 mansfield city schools ohio calendar