Adipocyte rna-seq
WebMay 20, 2024 · Whole transcriptome RNA-seq reveals key regulatory factors involved in type 2 diabetes pathology in peripheral fat of Asian Indians Aditya Saxena, Nitish Mathur, Pradeep Tiwari & Sandeep Kumar... WebMar 11, 2024 · For RNA-seq, RNA was extracted from about 30 mg of each individual adipose tissue. Equal amounts of RNA were pooled to obtain 6 independent replicates/conditions that were processed for RNA-seq. As depicted in Fig. S1D, each chromatin pool was paired with 3 RNA-seq pools (adipose tissue coming from the same …
Adipocyte rna-seq
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WebNov 7, 2024 · RNA was prepared from eight different cell WAT cell types (ASCs, total adipose tissue macrophages (ATMs), M1 ATMs, M2 ATMs, total T cells, CD4 + T cells, CD8 + T cells, and mature adipocytes). Ten nanograms of RNA was amplified using four cycles and loaded onto Clariom™D microarray chips. For details see Additional file 1: … WebDec 21, 2024 · Single nucleus sequencing (snRNAseq) is a modified version of scRNAseq, wherein a single nucleus is sequenced for actively transcribed RNA instead of a single cell. This technique is especially effective on tissues that cannot be dissociated into intact single cells, such as adipocytes.
WebApr 30, 2024 · Single-cell RNA-seq profiles of human preadipocytes during adipogenesis in vitro identifies at least two distinct classes of subcutaneous white adipocytes. These … WebWe analyzed adipose gene expression using RNA sequencing in the fenofibrate and omega-3 fatty acid modulation of endotoxemia (FFAME) trial of healthy individuals at three …
WebFeb 8, 2024 · RNA-seq was used to determine genes regulated by EGCG and identify the key differences between the two functionally distinct adipose tissues. Taken … WebSep 9, 2016 · Adipogenesis of primary brown preadipocytes In order to get a global view of gene expression during adipogenesis of brown preadipocytes, RNA-Seq was performed to identify the genes involved and their function. RNA was isolated from primary cultured brown preadipocytes (D0) and differentiated adipocytes (D7).
WebDec 3, 2024 · Transcriptome-wide m 6 A-Seq Reveals Global m 6 A Modification Patterns During Yak Adipocyte Differentiation. The yak adipocyte and preadipocyte of three biological replicates were used for transcriptome-wide m 6 A-sequencing (m 6 A-seq) and RNA-sequencing (RNA-seq) assays. In total, 12 libraries were sequenced, comprising …
WebMar 7, 2024 · Results: In this study, we found that brown adipose tissue (BAT) existed within the perirenal fat at 1 day after birth (D1) and transferred into white adipose tissue (WAT) … cursed optimus primeWebApr 11, 2024 · Background: Insulin resistance (IR) is a major contributing factor to the pathogenesis of metabolic syndrome and type 2 diabetes mellitus (T2D). Adipocyte metabolism is known to play a crucial role in IR. Therefore, the aims of this study were to identify metabolism-related proteins that could be used as potential biomarkers of IR and … chart stimulated recall worksheetWebG protein-coupled receptors (GPCRs) are expressed essentially on all cells, facilitating cellular responses to external stimuli, and are involved in nearly every biological process. Several members of this family play significant roles in the regulation of adipogenesis and adipose metabolism. However, the expression and functional significance of a vast … cursed orange and friday night funkinWebMar 16, 2024 · As expected, adipocytes were found only in the sNuc-seq dataset. The sNuc-seq data were also enriched for vascular cells and macrophages, probably because collagenase digestion did not fully... cursed opal necklaceWebEndometrial cancer (EC) is the fourth most common cancer in women, and half of the endometrioid EC (EEC) cases are attributable to obesity. However, the underlying … cursed orb rarityWebOct 23, 2024 · Additionally, single-cell RNA-sequencing (scRNA-Seq) of adipose stromal populations revealed an increase in IL10-producing adaptive immune cells under … charts that show percentagesWebNov 8, 2024 · The snRNA-seq data of digested adipocytes from inguinal WAT reveals a complex subpopulation of mature adipocytes with distinct genetic signatures [ 35 ]. Recently, snRNA-seq analysis of the WAT identified a rare subpopulation of adipocytes in mice that increase in abundance at higher temperatures. cursed orange